Crowley, D.J., research program has focused on elucidating mechanisms and regulation of DNA repair and DNA damage tolerance in the extremely halophilic archaeon, Halobacterium sp. NRC-1 and the moderately halophilic Haloferax volcanii. Projects of ongoing interest include modification and optimization of assay systems to study transcription-coupled repair of ultraviolet light-induced DNA lesions in Halobacterium sp. NRC-1. Also targeted knockouts of putative repair genes inHalobacterium for purposes of functional characterization. Twenty-five undergraduate students have participated in this research at Assumption College and Mercer University. Recent collaborators have included Dr. Shirley McCready at Oxford-Brookes University, Oxford, UK and Dr. Justin Courcelle at Portland State University, Portland, OR.
Dissertation Research, Stanford University, 1994-1999
Research in the laboratory of Dr. Philip Hanawalt. DNA damage inducible responses and mechanisms of DNA repair inEscherichia coli. Major focus on the genetic control of nucleotide excision repair genes and the effects of their regulation on the efficiency of repair of ultraviolet light induced DNA lesions. Additional studies characterizing modes of DNA repair in Haloferax volcanii.
Undergraduate Honors Thesis, Univ. of Massachusetts Medical Center, 1991-1993
Research in the laboratory of Dr. Michael Volkert. Development of an assay to measure transcription-coupled DNA repair of ultraviolet light irradiated phage lambda in its host, Escherichia coli. Also contributed to elucidating modes of DNA repair and mutagenesis in aerobic and anaerobically grown Escherichia coli treated with alkylating or oxidizing chemicals.
Undergraduate Research, College of the Holy Cross, 1991-1992
Research in the laboratory of Dr. George Hoffmann. Investigated the effect of monofunctional and bifunctional alkylating agents on the rates of mutation and recombination in Salmonella typhimurium.
Boubriak, I., Ng, W.L., DasSarma, P., DasSarma, S. Crowley, D.J., and McCready, S. 2008. Transcriptional responses to biologically relevant doses of UV-B radiation in the model archaeon, Halobacterium sp. NRC-1. Saline Systems. 4(1):13.
Crowley, D.J., Boubriak, I., Berquist, B.R., Clark, M.*, Richard, E.*, Sullivan, L.*, DasSarma, S. and McCready, S. 2006. The uvrA, uvrB, and uvrC genes are required for repair of ultraviolet light induced DNA photoproducts in Halobacterium sp. NRC-1. Saline Systems, 2(1):11. (“Recommended” by Faculty of 1000 Biology: http://www.f1000biology.com/article/id/1044118/evaluation)
Crowley, D.J. and Courcelle, J.C. 2002. Answering the call: Coping with DNA damage at the most inopportune time. Journal of Biomedicine and Biotechnology 2(2):66-74.
Hoffmann, G.R., Crowley, D.J. and Theophiles, P.J. 2002. Comparative potencies of induction of point mutations and genetic duplications by the methylating agents methylazoxymethanol and dimethyl sulfate in bacteria. Mutagenesis 17(5):439-444.
Crowley, D.J. and Hanawalt, P.C. 2001. The SOS-dependent upregulation of uvrD is not required for efficient nucleotide excision repair of ultraviolet light induced DNA photoproducts in Escherichia coli. Mutation Research 485(4):319-329.
Hanawalt P.C., Crowley D.J., Ford J.M., Ganesan A.K., Lloyd D.R., Nouspikel T., Smith C.A., Spivak G., Tornaletti S. 2000. Regulation of nucleotide excision repair in bacteria and mammalian cells. In: Cold Spring Harbor symposia on quantitative biology, Vol. 65: Biological Responses to DNA Damage. p. 183-191.
Courcelle, J., Crowley, D. J., Hanawalt, P.C. 1999. Recovery of DNA replication in UV-irradiated Escherichia coli requires both excision repair and RecF protein function. Journal of Bacteriology, 181(3):916-922.
Crowley, D.J. and Hanawalt, P.C. 1998. Induction of the SOS response increases the efficiency of global nucleotide excision repair of cyclobutane pyrimidine dimers, but not 6-4 photoproducts, in UV-irradiated Escherichia coli. Journal of Bacteriology, 180(13):3345-3352.
Zou, Y., Crowley, D.J., and Van Houten, B. 1998. Involvement of molecular chaperonins in nucleotide excision repair. Journal of Biological Chemistry, 273(21):12887-12892.
Sweder, K.S., Verhage, R.A., Crowley, D.J., Crouse, G.F., Brouwer, J. and Hanawalt, P.C. 1996. Mismatch repair mutants in yeast are not defective in transcription-coupled repair of UV-induced DNA damage. Genetics, 143:1127-1135.
Volkert, M.R., Loewen, P.C., Switala, J., Crowley, D*., Conley, M. 1994. The delta (argF-lacZ) 205(U169) deletion greatly enhances resistance to hydrogen peroxide in stationary-phase Escherichia coli. Journal of Bacteriology, 176(5):1297-1302.
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